Human DNA Typing Using PCR Kit

Human DNA Typing Using PCR Kit

Product Code : EL-GE-10503

Quick Answer: The Human DNA Typing Using PCR Kit lets 25 students use PCR primers for a 300 base pair Alu insertion on chromosome 16 (PV92) to determine their own genotype.

Last updated: October 2026

Key Features

  • For 25 students
  • PCR primers for the PV92 Alu insertion (300 base pairs) on chromosome 16
  • Students extract their own DNA and determine their genotype
  • Includes DNA buffers, proteinase K, agarose, TBE buffer powder, PCR beads, primer mix, DNA ladder and stains
  • Some components need freezer storage on receipt

Technical Specifications

SpecificationValue
Students25
Extraction50 minutes
PCR set-up10 minutes
PCR90 minutes
Electrophoresis60 minutes
Staining5 minutes to overnight
Not includedElectrophoresis tank with casting trays, micropipettes, power supply, thermal cycler, 2 x water bath, UV or blue light transilluminator, microcentrifuge, microwave or hot plate

About This Product

In this practical, students extract DNA from their own cells, amplify the PV92 region by PCR and run the products on an agarose gel. Whether the 300 base pair Alu insertion is present on one, both or neither copy of chromosome 16 gives each student's genotype, and the class results can then be compared.

Frequently Asked Questions

Q1. How long does each stage take?
Extraction takes 50 minutes, PCR set-up 10 minutes, PCR 90 minutes and electrophoresis 60 minutes. Staining takes from 5 minutes to overnight.

Q2. What equipment is needed besides the kit?
A DNA electrophoresis tank with accessories, micropipettes, a suitable power supply, a thermal cycler, two water baths, a UV or blue light transilluminator, a microcentrifuge and a microwave or hot plate.

Related: Genetics & Evolution, Identification of Genetically Modified Food Kit, Gene Regulation Kit, Plant Culture Set.

For a quotation on the Human DNA Typing Using PCR Kit, contact School Equipments India.

   
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